Treatment of macrophages with SMT inhibits LPS-induced NO production but not apoptosis.
(A) The expression of iNOS was measured by Northern blot in macrophages treated with 100 ng/mL of LPS in the presence or absence of SMT, an iNOS inhibitor (20 μmol/L). (B) SMT inhibits LPS-induced NO production; 106 macrophages were treated with 100 ng/mL of LPS for the indicated times in the presence or absence of SMT (20 μmol/L). The production of NO was assessed by determination of the nitrate/nitrite levels. (C) SMT did not inhibit LPS-induced apoptosis. The cells were treated with 100 ng/mL of LPS for the indicated times in the presence or absence of SMT (20 μmol/L). Each experiment was performed in triplicate, and the results of 1 representative of 2 independent experiments are represented as the mean value ± SD.
