LPS-induced apoptosis in macrophages from iNOS KO mice.
(A) LPS induces TNF-α expression in iNOS KO macrophages. The expression of iNOS and TNF-α mRNA was analyzed by Northern blotting. (B) LPS induces TNF-α expression in iNOS KO macrophages. The secretion of TNF-α was analyzed by ELISA in macrophage cultures from each group of mice. Each experiment was performed 3 times and represented as the mean value ± SD. (C) LPS induced similar rates of apoptosis in macrophages from iNOS KO and in control macrophages. Macrophages (105) from control and KO mice were treated with LPS (100 ng/mL) for the indicated periods of time in the presence or absence of SMT (20 μmol/L). Apoptosis was determined by ELISA. Each experiment was performed in triplicate and represented as the mean ± SD.
