Fig. 2.
Expression of the Bcl2 family proteins in HTLV-II–transformed and HTLV-II–immortalized cell lines.
Total proteins (50 μg) were resolved on 12.5% SDS-PAGE and transferred onto PVDF membranes and probed with specific antibodies for Bcl2, Bcl-XL, Bax, Bak, and BAD. Comparable protein loading was verified using an antibody specific for the housekeeping gene product β-tubulin. Results are representative of 2 independent experiments using uninfected PBMCs from 2 separate donors.