Fig. 5.
Fig. 5. Overexpression of Bcl-XL in uncultured acute T-cell lymphocytes from ATLL patients. / Lymphocytes from uninfected donors and ATLL patients were isolated by Ficoll-Hypaque gradient, washed, and lysed in RIPA buffer. Total proteins (50 μg) were separated on 12.5% SDS-PAGE transferred onto PVDF membranes and probed with specific antibodies for Bcl2, Bcl-XL, and Bax. Comparable protein loading in each lane was verified using an antibody specific for the housekeeping gene product β-tubulin. Results are representative of 2 independent experiments using 2 separate donors for PBMCs.

Overexpression of Bcl-XL in uncultured acute T-cell lymphocytes from ATLL patients.

Lymphocytes from uninfected donors and ATLL patients were isolated by Ficoll-Hypaque gradient, washed, and lysed in RIPA buffer. Total proteins (50 μg) were separated on 12.5% SDS-PAGE transferred onto PVDF membranes and probed with specific antibodies for Bcl2, Bcl-XL, and Bax. Comparable protein loading in each lane was verified using an antibody specific for the housekeeping gene product β-tubulin. Results are representative of 2 independent experiments using 2 separate donors for PBMCs.

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