Heterogeneous cellular expression of FANCC.

Cells were stained with monoclonal anti-FANCC antibody and a secondary Oregon green–labeled goat antimouse antibody. Fluorescence was observed using a confocal imaging system. A single slice through the plane of cells is displayed. (A) 293 NEO cells. No staining of endogenous FANCC is detectable. (B) 293 FANCC E2 cells. In this field, 1 cell has a large amount of cytoplasmic FANCC, whereas an adjacent cell is only weakly positive. (C) 293 FANCE2 cells. Some cells have only cytoplasmic FANCC, others have predominately nuclear FANCC, and some have FANCC in both compartments. Nuclear foci are clearly visible in the top and bottom cells. In addition to the subcellular localization differences, there is marked cell-to-cell variation in total cellular FANCC expression.