Proteasome inhibitors MG132 and lactacystin increase FANCC expression in cells synchronized at the G1/S boundary.

293 FANC E2 cells were treated for 24 hours with complete growth medium (Control) or 1.3 mmol/L HU (HU). HU-synchronized cells were treated with vehicle only (DMSO), 20 μmol/L MG132 (MG132), or 20 μmol/L lactacystin (LACT.) for an additional 6 hours. Whole-cell extracts were analyzed for FANCC expression by Western blotting and probing with a monoclonal anti-FANCC antibody (8F3).