Fig. 6.
Fig. 6. Maturation of IIbLeu262Proβ3is delayed in COS-7 cells. / COS-7 cells transfected with wild-type (WT) αIIb and either WT β3 or Leu262Proβ3 (LD) were pulsed with sulfur 35–methionine for 30 minutes and chased for up to 22 hours with medium containing 1 mg/mL cold methionine. Cell lysates prepared at the indicated times were immunoprecipitated with an anti-β3 antibody (AP3) and analyzed by autoradiography of an SDS–7% polyacrylamide gel run under reducing conditions. Although preαIIb and Leu262Proβ3 synthesis and association are normal in lysates from LD, the appearance of a mature αIIbLeu262Proβ3 complex is markedly delayed.

Maturation of IIbLeu262Proβ3is delayed in COS-7 cells.

COS-7 cells transfected with wild-type (WT) αIIb and either WT β3 or Leu262Proβ3 (LD) were pulsed with sulfur 35–methionine for 30 minutes and chased for up to 22 hours with medium containing 1 mg/mL cold methionine. Cell lysates prepared at the indicated times were immunoprecipitated with an anti-β3 antibody (AP3) and analyzed by autoradiography of an SDS–7% polyacrylamide gel run under reducing conditions. Although preαIIb and Leu262Proβ3 synthesis and association are normal in lysates from LD, the appearance of a mature αIIbLeu262Proβ3 complex is markedly delayed.

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