_vLeu262Proβ₃ on 293 cells retracts a fibrin clot.

Untransfected 293 cells (solid circles) and cells expressing WT α_vβ₃ (solid squares) or α_vLeu262Proβ₃ (open circles) were assessed for their ability to retract clotted fibronectin-depleted plasma (A) or purified fibrinogen (B). The estimated clot volume is expressed as a function of time (minutes). The data shown are representative results from 3 separate experiments. α_vLeu262Proβ₃-mediated clot retraction was comparable to that of WT α_vβ₃. (C) WT α_vβ₃ cells (open columns) or α_vLeu262Proβ₃ cells (solid columns) were incubated for 15 minutes at 22°C with peptides RGDW, RGEW, or H12 (all at a final concentration of 2 mmol/L) or with the indicated antibodies (final concentration, 10 μg/mL). Only RGDW and the α_vβ₃-specific antibody LM609 fully inhibited clot retraction.