_vLeu262Proβ₃ does not support cell adhesion to immobilized fibrinogen.

Untransfected 293 cells (open columns) and cells expressing WT α_vβ₃ (hatched columns) or α_vLeu262Proβ₃ (solid columns) were labeled with calcein am and allowed to adhere to immobilized substrates (bovine serum albumin, AP3, fibronectin, or fibrinogen) for 1 hour at 37°C. Adhesion is shown as the ratio of fluorescence per well before and after washing. Each column represents the mean (± SD) value from triplicate samples, and the results are representative of 4 separate experiments.