ASO–PCR quantitation of malignant cells.

Quantitation of malignant cells in samples from myeloma patients H and 6 using real-time IgH ASO–PCR (A-C) or conventional ASO–PCR and densitometric scanning (D). Calibration curves were constructed by plotting the initial number of tumor cells against the Ct (B), the amount of emitted fluorescence (PCR product) at 35 and 50 cycles (C), or the density (D).