PCR and PCR–SSCP analyses.

(A) Analysis of PCR products of BCL10 exon 3.2 on polyacrylamide gels. M, marker; N, normal DNA samples; −Ve, negative control. Patients C2, C10, and C11 showed a novel PCR product corresponding to a 17-bp deletion, whereas patients C5 and C13 showed a novel PCR band corresponding to a 28-bp deletion. (B) PCR–SSCP analysis of BCL10 exon 3.1 (left panel) and exon 3.2 (right panel). N, normal DNA samples; C, DNA samples from patients with lymphoma. Open arrowhead indicates a G > A polymorphism in codon 213. Filled arrowhead highlights abnormal migrating SSCP bands.