Western blot analysis.

Patient C13 had a 28-bp deletion between codons 170 and 179 that caused a truncated BCL10 of 169 amino acids. Western blot analysis of this case shows 3 truncated forms of BCL10. The biochemical differences of these various forms are unclear, nor is it clear why the amount of the truncated protein is much less than that of the full-length BCL10 despite the prevalence of tumor cells in the fresh tissue examined. Patient C4 had a substitution at the 3′ coding exon 2 splicing site, which led to a truncated BCL10 product of 119 amino acids that was not recognized by the monoclonal antibody 151 (mapping to amino acids 122-168). Both this case and tonsil show only full-length BCL10.