Fig. 5.
Sequence of events regarding real-time E2-BSA–stimulated [Ca2+]i transients and NO release from human granulocytes.
Addition of 10−9 mol/L E2-BSA to the medium results in immediate calcium transients (application added at the base of the steep increase) that are then followed by a progressive decrease lasting about 2 minutes. Approximately 40 seconds later, an increase in NO release (peak level, 13 nmol/L E2-BSA) occurs and lasts for 10 minutes. The mean values (n = 4) were graphed with spline curves so that the precise times could be better visualized.