ER gene expression in human monocytes and neutrophils.

RT-PCR was performed using either no RNA (negative control, lane 1) or 3 μg RNA from human breast cell lines (positive control, lane 2), monocytes (lane 3), or neutrophils (lane 4). MCF7 and MDA MB231 cell lines were used for ERα and ERβ amplification controls, respectively. PCR amplification was also performed using a primer pair specific for human GAPDH as a cDNA control. The PCR products (one-fifth of the ERα and ERβ amplification, one-tenth of the GAPDH amplification) were separated by electrophoresis on a 2% agarose gel. DNA markers (1-kb ladder) were run in parallel. The sizes of the amplified products are indicated in the base pair on the right.