Alignment of mouse and human ESOP-1 and mESOP-1 expression.

(A) Alignment of amino acid sequences of murine and human ESOP-1. Identical residues are indicated by asterisks. Putative cleavage site by the signal peptidase is indicated by an arrowhead.17 The 7 cysteine residues conserved are boxed. (B) mESOP-1 mRNA expression during mouse embryogenesis. Clontech mouse embryo multiple-tissue Northern blot was probed with mESOP-1 cDNA. (C) mESOP-1 expression among adult mouse organs; 20 μg total RNA was isolated from mouse tissues and blotted onto the nylon membrane, then hybridized with labeled mESOP-1 cDNA. The expression of mESOP-1 mRNA in adult tissues was detected strongly in spleen, bone marrow, decidua of 8.5 dpc, liver, kidney, and weakly in thymus, ovary, testis, small intestine, and skin.