AG-490 inhibits IL-4–induced proliferation in both D10 and CT.4S cells in a dose-dependent manner.

Proliferation of quiescent D10 (A) or CT.4S (B) cells (5 × 10⁴/well) was examined after treatment with the DMSO control or increasing concentrations of AG-490 (ordinate) for 16 hours at 37°C in the presence (solid box) or absence of 1 nmol/L IL-4 (hatched box). Cells were then pulsed with tritium-thymidine (18.5 × 10² Bq/200μL) for 4 hours. Incorporation of radiolabeled probe is plotted on the abscissa, expressed as total counts per minute (n = 6).