AG-490 ablates IL-4–induced transcriptional activity of STAT6 binding element of a luciferase reporter construct.

D10 cells were stably transfected with a 3 copies of a STAT6 binding element–pGL3 promoter–luciferase construct or with pGL3 promoter–luciferase construct alone. Cells were then treated in the absence or presence of AG-490 for 16 hours and incubated with or without IL-4 (100 nmol/L) for 10 hours. Luciferase activity of lysed cells was measured and normalized against protein concentration.