Activation of JunD in HTLV-I-infected T-cell lines.

(A) Nuclear extracts of the ATL-derived cell line, MT-1, contain AP-1 binding activity, involving JunD. The antisera indicated above lanes were incubated with nuclear extracts from MT-1 cells and subjected to EMSA using IL-8 AP-1 as a probe. A supershift band was observed on addition of antisera against JunD (arrow). (B) Expression of JunD mRNA determined by Northern blot analysis of HTLV-I-infected T-cell lines. GAPDH expression was used as a control.