Fig. 3.
AICD and CD3-modulation by native SPV-T3a.
The EBNA3C-reactive CTL clone was incubated with 10−8mol/L MoAb SPV-T3a, or isotype-matched control MoAb MOPC-141, for 24 hours at 37°C. Subsequently, cells were washed and cultured for another 72 hours in culture medium. The number of surviving cells was then determined by viability staining and flow cytometric analysis, and expressed as percentage relative to the untreated control (A). Membrane expression of free and SPV-T3a-bound CD3-antigen was determined as described in Materials and methods and indicated as percentage relative to control cells (B). Data represent the mean ± SD of 3 experiments performed in triplicate.
