Effect of IT treatment on NK activity.

PBMC were treated with 10⁻⁸ mol/L WT1-dgA (▪) or SPV-T3a-dgA (▴) or without IT (□), for 24 hours at 37°C. After treatment, cells were washed and cultured for an additional 3 days in culture medium without IT. Subsequently, remaining NK activity was determined by specific lysis of ⁵¹Cr-labeled K562 blasts and expressed as percentage relative to untreated cells. During the experiment, 50 U/mL recombinant IL-2 was added to the culture medium to increase NK activity. Data represent the mean ± SD of 3 experiments performed in triplicate.