Transfection of HEK 293 with Mac-1.

Flow cytometry analysis and FACSorting of HEK 293 cells transfected with Mac-1 (A to D). (A) Immunofluorescence assay using mAb LM2/1 with control transfected HEK 293 cells as a control. (B) Immunofluorescence assay using mAb LM2/1 with Mac-1 transfected HEK 293 cells. (C) Low level of expression of Mac-1, after FACSorting of Mac-1 transfected HEK cells. (D) High level of expression of Mac-1, after FACSorting of Mac-1 transfected HEK cells.