Effect of HKa on cellular adhesion to fibrinogen and ICAM-1.

HEK 293 cells transfected with Mac-1 were labeled with CMFDA, added to wells precoated with the ligands ICAM-1 (▾), fibrinogen, (○) or fibronectin (•), and incubated in the presence or absence of indicated HKa for 60 minutes. As a control, untransfected HEK 293 cells labeled with CMFDA were added to fibrinogen-coated (▪) or ICAM-1–coated (□) wells. After they were washed 3 times, adherent cells were lysed. Plates were read on a Cytofluor 2350 system. Percentage of adherent cells to each ligand in the presence of HKa was determined by comparing that with in the absence of HKa, which was calculated as 100%. Data are the mean ± SE of 3 separate experiments.