Fig. 4.
FACS analysis obtained 3 days after gene transfer.
Cells were stained with anti-CD3, anti-CD4, or anti-CD8 and anti-NGFR (monoconal antibody 20.4). The results indicate an equal transvection of CD4 and CD8 lymphocyte subsets, reflected by the CD4/CD8 ratio of 1.6 in both the transduced NIT+ T cells and the nontransduced NIT− T-cell fraction.