Fig. 4.
![Fig. 4. Neutralization of endogenous IL-10 during LPS priming completely ameliorates the ET-associated suppression of T-cell IFN-γ production but not the suppression of T-cell proliferation in response to different protein recall antigens. / PBMCs from 2 donors were cultured for the first 24 hours with (2 ng/mL) or without LPS in the presence of either a control murine IgG1 or anti-IL-10 MoAb and recultured with either 2.5 μg/mL candidin (Can), 0.08 IU/mL tetanus toxoid (TT), 5 PPD-S/mL tuberculin (Tbc), or without stimulation. (A) Cellular [5′-3H]thymidine incorporation after 3 days of antigen stimulation is given from experiments in triplicate assays (mean ± SEM). (B) IFN-γ production after 4 days of antigen stimulation was assessed by ELISA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.218/4/bloo01304004x.jpeg?Expires=1735620066&Signature=TKDXhkv12siXmSxU2-zQ5uXmdosWjApyG7vlvIJz-VVd6Quc1jrzB4zl-PsGyRF1jqEYTGox8nDZFcCKn5RpQy9eZFLBi~NxW15vNVM7bR7BgAj8X4srQDuZIegFFGzKMg7QwS1kUsRJyo~1t7YYFZRVBTh3kafBevKXnJxj6wBQSOFXFzcJjRkWuXPxLaDCQzH05kMk9SFiDbNqWuTUwmi9TmQPKKX8BGTLGG7oO-gWWlp0SpwRsKNBYInLSrK7JtWS~8Mphd7WRK68Wil8YyEeoktYHry9aeaoprktdD3fEDBx1~b5nKU~de9XwHduWlJ8C~0iU5Co8-opaCvZbA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Neutralization of endogenous IL-10 during LPS priming completely ameliorates the ET-associated suppression of T-cell IFN-γ production but not the suppression of T-cell proliferation in response to different protein recall antigens.
PBMCs from 2 donors were cultured for the first 24 hours with (2 ng/mL) or without LPS in the presence of either a control murine IgG1 or anti-IL-10 MoAb and recultured with either 2.5 μg/mL candidin (Can), 0.08 IU/mL tetanus toxoid (TT), 5 PPD-S/mL tuberculin (Tbc), or without stimulation. (A) Cellular [5′-3H]thymidine incorporation after 3 days of antigen stimulation is given from experiments in triplicate assays (mean ± SEM). (B) IFN-γ production after 4 days of antigen stimulation was assessed by ELISA.
