Fig. 4.
![Fig. 4. CD3 stimulation of PBTs 24 hours after CD4XL down-modulates Bcl-x in both CD4 T cells and CD8 T cells, and CD28 costimulation prevents Bcl-x down-modulation. / [CD4XL/CD3] PBT cell cultures are showing Bcl-x expression in CD4 T cells (A) and in CD8 T cells (B). Cell-culture conditions are indicated in each panel, and isotype controls are indicated in top panels. Cells were analyzed by flow cytometry for intracytoplasmic Bcl-x expression in conjunction with surface labeling with FITC-anti-CD8 mAb. Broken lines represent the lowermost boundary of cells cultured in medium alone and labeled with anti–Bcl-x Ab. Cells that emerged to the left of this line represent Bcl-x down-modulated cells, and their percentages are indicated in the figure.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.195/4/bloo01351004x.jpeg?Expires=1735583591&Signature=J1Tfu9ewrJtzOAAfHKzVgq9l9~kj3gkRnECWRlY3A30S7ys3NlELcXza0YJutllZVcMrfSmuWY9K4SA5IttrnmgwSOPzAG79iDJrWyRo7tNhxUJcsR1Sezz9S9QN~7ND3woZda1jap2xkAT3O7rAKp1w6mV9Mx4pJJSr5i6a2CIUOwqm6j9P9Yy2aLVL9uniVDKlNzNONLQN~CktKqHJxiz6zrrpQmDphLMGLY~VdhDdC3rE7Uzl4AavXb8nD9MB1X7W~Wf8StApIGS6APwAmtpBg5g-~OwM7wipb6oBXq6AQClx54x7vcANae~HFdso1Y7AqoVC9mxA1vRs5ALDNQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD3 stimulation of PBTs 24 hours after CD4XL down-modulates Bcl-x in both CD4 T cells and CD8 T cells, and CD28 costimulation prevents Bcl-x down-modulation.
[CD4XL/CD3] PBT cell cultures are showing Bcl-x expression in CD4 T cells (A) and in CD8 T cells (B). Cell-culture conditions are indicated in each panel, and isotype controls are indicated in top panels. Cells were analyzed by flow cytometry for intracytoplasmic Bcl-x expression in conjunction with surface labeling with FITC-anti-CD8 mAb. Broken lines represent the lowermost boundary of cells cultured in medium alone and labeled with anti–Bcl-x Ab. Cells that emerged to the left of this line represent Bcl-x down-modulated cells, and their percentages are indicated in the figure.
