Fig. 6.
![Fig. 6. [CD4XL/CD3] treatment of PBTs results in induction of TNF and IFNγ in T cells undergoing apoptosis; anti-CD28 stimulation results in IL-2 expression and rescues T cells from apoptosis. / PBTs (1 × 106/mL) were cultured in complete medium, or treated with CD4XL, and were stimulated after 24 hours with anti-CD3 mAb with or without CD28-stimulation in the presence of Brefeldin A. Following a culture period of 18 hours, cells were harvested and labeled with annexin V and anticytokine antibodies for simultaneous assessment of intracytoplasmic cytokine production and apoptosis in CD4 T cells (A) and in CD8 T cells (B). Percentages of positive cells are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.195/4/bloo01351006x.jpeg?Expires=1735584970&Signature=FLYDrYGG~yHUnod8Gc466NHxk0JBdaAGU0p6g2Eed0nQ3me5KjZ~YIwmKQVXYEjGN0SPEIm6bL53tzpEzS6mrqsmb8oMJLRxDeXdvG982lc8~HwhjsDIUCsXdL9L49sKokPY9JIgigqgNsu-91CcX4dikx1RQz8x8QIqezJMneKHkRdP1RBZhowOi~8tGp7DRO9cgb-Sa8JYgPH7h0CIeOAv7NuFRZMGAcKp7YkD14TF1TRLAIJWhKMq~KEo0azlBs9pUGnbZqrBPZAe6OANv3wvJBwBYWYLeX5~pON9YVeEf-0N1s9B6Z6IfT07eR7HRMUl4VDp2ueJEb5jilC8yQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
[CD4XL/CD3] treatment of PBTs results in induction of TNF and IFNγ in T cells undergoing apoptosis; anti-CD28 stimulation results in IL-2 expression and rescues T cells from apoptosis.
PBTs (1 × 106/mL) were cultured in complete medium, or treated with CD4XL, and were stimulated after 24 hours with anti-CD3 mAb with or without CD28-stimulation in the presence of Brefeldin A. Following a culture period of 18 hours, cells were harvested and labeled with annexin V and anticytokine antibodies for simultaneous assessment of intracytoplasmic cytokine production and apoptosis in CD4 T cells (A) and in CD8 T cells (B). Percentages of positive cells are indicated.
