Fig. 8.
![Fig. 8. Cell-to-cell contact is required for [CD4XL/CD3]-activation-induced CD8 T-cell apoptosis. / CD4 T cells treated with (right panels) or without (left panels) CD4XL were placed below the insert (bottom chamber), and CD8 T cells were placed in the upper chamber. After 24-hour culture, anti-CD3 mAb was added and cells were incubated for an additional 18 hours. Cells were harvested, labeled with annexin V, and stained for intracytoplasmic IFNγ. Percentages of positive cells in each quadrant are indicated. Data represent 1 of 2 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.195/4/bloo01351008x.jpeg?Expires=1735584531&Signature=FdkSesUoEkhJrSu~Ay2mIxej7an9xB5noDWf1R0pFAiMv7SqppVq975LdEtv~hWUhacnkPhRGM7Xu4-R9dtskZzUvrD7bxgDzvWgpvR-qFjfirhZ0wfvySL0Qghq2FU-jxc2TlDNNO-2NUsn~qwzYhOmjFL4EiYmscCRU2UdiGXb47mG7raQDh-JSvavYgiIHsijwSDBmhsfUd0N835M3O7Hg-JyE7TKvhpElxGOdpX-u2SzgxgEOWnLNQDZg4olVE4swnLLKqfD9oukm7uc9Uynoe~WHM4GVTe97y4deAQEupKS4lnkCSFTmr94Di1iJ6pEIY5sUYWLWTkFkQb8-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell-to-cell contact is required for [CD4XL/CD3]-activation-induced CD8 T-cell apoptosis.
CD4 T cells treated with (right panels) or without (left panels) CD4XL were placed below the insert (bottom chamber), and CD8 T cells were placed in the upper chamber. After 24-hour culture, anti-CD3 mAb was added and cells were incubated for an additional 18 hours. Cells were harvested, labeled with annexin V, and stained for intracytoplasmic IFNγ. Percentages of positive cells in each quadrant are indicated. Data represent 1 of 2 experiments.
