Effect of IFN-γ on activation of caspase3.

Data show typical results of 3 independent experiments. Cells were incubated with or without rhEPO (10 U/mL) or rhIFN-γ (1000 U/mL), or both, in serum-free medium for 16 hours. Cells were then incubated with caspase3 substrate solution GDEVDGI for 1 hour at 37°C, and flow cytometric analysis was performed. The open histogram shows the fluorescence of day 7 ECFCs as control. Each panel shows data on cells incubated without rhEPO and rhIFN-γ (A), with rhEPO alone (B), with rhIFN-γ alone (C), and together with rhEPO plus rhIFN-γ (D).