Effect of fzr overexpression on tumor formation.
(A) Western blot analysis of DAC and MV cells retrovirally infected with murine stem cell virus (MSCV) retrovirus produced from empty vector control (“vector”) or fzr constructs (“fzr”). Expression was tested in the in vitro cell lines (“cells”) and in cells obtained from tumors (“tumors”) using anti-Fzr rabbit serum. Equal protein loading was verified by Ponceau S staining. (B, C) Balb/c mice were injected IP with vector-only or fzr infectants of DAC (B) or MV (C) at 5 × 105. Thirteen mice were injected for each of the 4 groups and monitored for 50 days. Mice were killed upon onset of disease or at the end of the monitoring period to confirm tumor formation and collect tissues, and all mice were killed after the monitoring period.
