Phenotypic expression, redirected cytoxicity, and proliferative activity.

Phenotypic expression (A), redirected cytotoxicity against P815 target cells (B), and proliferative activity (C) in the presence of mAbs to CD30, CD30L, CD40, CD40L, CD70, CD95, and CD95L in a representative CD3⁺TCRαβ⁺ patient (case 11). Because the expression of TNF-R and TNF-L antigens was different among the patients, the relationship among phenotype, cytotoxicity, and proliferation are shown for a correct interpretation of data. The differences between the peaks of cell fluorescence (continuous lines) with respect to controls (dotted lines) were analyzed using the Kolmogorov-Smirnov test for analysis of histograms and a D/s value ≥ 15 was accepted as significant, as reported in “Patients, materials, and methods.” Cytotoxicity and proliferation experiments were performed in triplicate and mean ± SEM are shown.