Fig. 4.
RT-PCR analysis of the expression of CD30, CD30L, CD40, CD40L, Fas, FasL, and control β-actin mRNA in GLs from 6 LDGL patients.
Lane 1 indicates molecular weight marker (100 base pairs [bp] DNA ladder); lane 2: control peripheral blood mononuclear cells stimulated for 12 hours with PHA (5 μg/mL) (positive control) for CD30, CD30L, CD40L, and FasL; positive control for CD40 was represented by purified B lymphocytes; lane 3: the negative control (sample without cDNA), lanes 4 to 6: 3 CD3+ LDGL cases (cases 3, 8, and 13 in Table 1), lane 7 to 9: 3 CD3− LDGL (cases 15, 17, and 20 in Table 1).