NF-E2 interacts directly with endogenous HS2 in K562 cells.

(A) Diagram of the human β-globin locus. Circles and rectangles represent DNaseI hypersensitive sites and the β-globin genes (ε, embryonic; Gγ and Aγ, fetal; and β, adult), respectively. The tandem MAREs within HS2 are indicated. (B) Ethidium bromide-stained agarose gels of input chromatin (left panel) and PCR products using primers specific for HS2, the Aγ-globin promoter, and IgH from a representative experiment. The immunoprecipitation conditions are indicated below each lane. No antibody, No Ab; anti-p45 polyclonal sera, p45; preimmune sera, PI; no chromatin, No Chr. (C) Relative intensity of PCR products from 5 independent experiments (mean ± SEM). The signal obtained with 0.02% of input was set to 1.