Fig. 2.
Fig. 2. NF-E2 interacts with HS2 of a stably integrated reporter construct. / (A) Structure of the HS2 (7.3)γluciferase reporter construct stably integrated into K562 cells. HS2 was separated from the Aγ-globin promoter by 2phage lambda MluI fragments (5.1 λ and 2.2 λ). (B) Ethidium bromide-stained agarose gels of input chromatin (left panel) and PCR products of samples using primers specific for HS2, the Aγ-globin promoter, and IgH from a representative experiment. The immunoprecipitation conditions are indicated below each lane. No antibody, No Ab; anti-p45 polyclonal sera, p45; preimmune sera, PI; no chromatin, No Chr. (C) Relative intensity of PCR products from at least 4 independent experiments (mean ± SEM). The signal obtained with 0.02% of input was set to 1.

NF-E2 interacts with HS2 of a stably integrated reporter construct.

(A) Structure of the HS2 (7.3)γluciferase reporter construct stably integrated into K562 cells. HS2 was separated from the Aγ-globin promoter by 2phage lambda MluI fragments (5.1 λ and 2.2 λ). (B) Ethidium bromide-stained agarose gels of input chromatin (left panel) and PCR products of samples using primers specific for HS2, the Aγ-globin promoter, and IgH from a representative experiment. The immunoprecipitation conditions are indicated below each lane. No antibody, No Ab; anti-p45 polyclonal sera, p45; preimmune sera, PI; no chromatin, No Chr. (C) Relative intensity of PCR products from at least 4 independent experiments (mean ± SEM). The signal obtained with 0.02% of input was set to 1.

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