Anticoagulant activity of APC single-point mutants.

The anticoagulant activity of the autolysis loop single mutants was tested in an APTT assay as described in “Materials and methods” over a range of APC concentration from 1.6 to 6.3 nmol/L. (A) APC: wild-type (+), 306A (▵), 307A (○), 308A (◊), 309A (□). (B) APC: wild-type (+), 311A (▵), 312A (○), 314A (◊). All curves are averaged from 4 to 8 individual experiments.