Flow-cytometric detection of Bcl-2 (A) and Bax (B) antigens in untreated and IL-7-treated leukemic cells from a patient with cortical T-ALL.

Blast cells were fixed, permeabilized, and stained by either direct or indirect immunofluorescence for Bcl-2 (mouse monoclonal antibody) or Bax (rabbit polyclonal antibodies against Bax peptide sequences), respectively. Solid lines correspond to antigen staining in untreated and IL-7-treated cells (bold and thin solid lines, respectively). Dotted lines correspond to background fluorescence of cells stained either with irrelevant antibodies (A and B) or with Bax antibody blocked with an excess of the corresponding specific peptide (B). Expression changes representative of T-ALL responsive to IL-7 are shown.