Fig. 5.
Correlation between IL-7–induced changes of Bcl-2 (A) and Bax (B) expression levels and extent of IL-7–specific apoptosis inhibition (▵NIL-7).
Changes of Bcl-2 but not Bax positively correlated with the anti-apoptotic effect of IL-7. IL-7–specific relative changes of expression of Bcl-2 and Bax were calculated from the ratio of antigen expression levels in IL-7–treated and untreated cells (RCEIL-7 = MESFIL-7/MESFmedium). Bcl-2 and Bax expression were assessed by flow cytometry as described in Figure 3 and were quantified in MESF units using calibration beads as fluorescence standards.
