Fig. 4.
![Fig. 4. Inhibition of protease activity. / (A) Bone marrow cells were aliquoted into 6 different tubes containing RIPA and the indicated inhibitors (50 μg/mL elastatinal, 0.5 mg/mL alpha-1-antitrypsin, 5 mmol/L EDTA, and leupeptin/Pefabloc/pepstatin/aprotinin [LPPA]). A lysate of35S-methionine–labeled blood cells (300 μL each) was added, and the reaction was immunoprecipitated for 3 hours at 4°C before gel separation. (B) The experiment was repeated with varying amounts of alpha-1-antitrypsin (0.1, 0.25, 0.5, and 1 mg/mL, shown in lanes 3-6, respectively). 35S-methionine–labeled bone marrow cells were lysed in RIPA with or without 0.5 mg/mL alpha-1-antitrypsin followed by immunoprecipitation for 1 hour at 4°C without any preclearing (lanes 7 and 8).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/2/10.1182_blood.v96.2.740/5/bloo01442004w.jpeg?Expires=1735543556&Signature=SZ0oZuAPAWrnwtt4ZPSYa34UgRMor~JVdjgvLrWuwBs~uihT1iLlIIDXucm-txS56sUyD-LnLTPd5wQubJh11wly01F7S6lx1cMBWf-7DQM3i~o8iSZSj9UJhuREgI360SmfgP1wdvjfFdm-3ExyIcShK8K39v7HXTB4MjhrseoGEGGvSeZ0BifYGwEmbtwLys-keOivjC0NUYn3rfTwOhZll5L6w5WJcF0vMCQhyrBwBEjaipS6WOWgQ5metWfNOHYUPc79OIa4NfX0v2FMsHSfWkY6IQxkhUeVan3ijWQUH3n1n9jD6qVML4BGRkSOoE6U25RP21~KDV8Lzi5p9Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of protease activity.
(A) Bone marrow cells were aliquoted into 6 different tubes containing RIPA and the indicated inhibitors (50 μg/mL elastatinal, 0.5 mg/mL alpha-1-antitrypsin, 5 mmol/L EDTA, and leupeptin/Pefabloc/pepstatin/aprotinin [LPPA]). A lysate of35S-methionine–labeled blood cells (300 μL each) was added, and the reaction was immunoprecipitated for 3 hours at 4°C before gel separation. (B) The experiment was repeated with varying amounts of alpha-1-antitrypsin (0.1, 0.25, 0.5, and 1 mg/mL, shown in lanes 3-6, respectively). 35S-methionine–labeled bone marrow cells were lysed in RIPA with or without 0.5 mg/mL alpha-1-antitrypsin followed by immunoprecipitation for 1 hour at 4°C without any preclearing (lanes 7 and 8).
