Stimulation of OCL formation.
(A) Effects of bone marrow plasma from controls and patients with MM on OCL formation in human marrow cultures. Bone marrow plasma and marrow cultures were processed as described in “Materials and methods.” Results represent the mean ± SEM for all assays done in quadruplicate. Bone marrow plasma from patients with MM significantly stimulated the formation of OCLs in human bone marrow cultures when compared to controls (47.75 ± 6 versus 28.25 ± 5 multinucleated cells/105 cells plated, respectively,P < .05). (B) Correlation of OCL formation induced by marrow plasma from 12 patients with MM and their respective levels of β2 microglobulin. Bone marrow plasma and marrow cultures were processed as described in “Materials and methods.” The relative levels of OSF positively correlated with β2microglobulin levels in patients with MM with normal renal function (correlation coefficient = 0.708).
