Reverse transcription-polymerase chain reaction (RT-PCR) analysis of MIP-1 mRNA expression in patients with MM

. Cycle-dependent RT-PCR was performed for 16, 20, or 24 cycles (lanes 1, 2, and 3) as described in “Materials and methods.” Results represent the mean ± SEM for 3 determinations for each patient. MIP-1α mRNA expression in patients with stage III MM was increased approximately 2- to 5-fold compared to controls in 3 of 4 patients with MM. A similar pattern of results was seen in 2 independent experiments.