Western blot analysis.

Integral membrane proteins (pH 11.5) from red blood cells, testis, or skeletal muscle were reduced, separated by SDS-PAGE, blotted, and detected with polyclonal antibody to Kell (left panel) or with a monoclonal antibody that recognizes glycophorins A and B (right panel). (Left panel) A single 93-kD band (arrow), the molecular size of Kell, was detected in red blood cells, testis, and skeletal muscle. (Right panel) Multiple bands corresponding to glycophorins A and B were detected in red blood cells but not in testis or skeletal muscle.