AFL4 treatment inhibits tumor angiogenesis.

Tumor-bearing regions were photographed on day 14 after tumor transplantation. Gross appearance of representative vascularization of control (A) and AFL4-treated (B) mice. Arrows and arrowheads indicate the vascular trunks governing tumor blood supply and those of the tumor-free side, respectively. Note that the size of the trunk is larger on the tumor-bearing side than on the other side. Such a dilatation is not clearly seen in the AFL4-treated mouse. (C, D) Higher magnification than that in A and B, respectively. Secondary and tertiary branches are poorly developed in the AFL4-treated mouse. Note the many micro-hemorrhages in this AFL4-treated tumor (arrows). Asterisks indicate tumors.