Selective outgrowth of Vγ9Vδ2 T cells on stimulation with IPP or pamidronate.

Representative 2-color FACS analysis of PBMC after 7-day culture in the presence of medium, IPP (4 μmol/L), or pamidronate (4 μmol/L) using FITC anti-γδ TCR and PE anti-CD3 mAb (A). Expansion of γδ T cells in 7-day primary PBMC cultures of 6 different healthy donors by IPP (40 μmol/L) or pamidronate (40 μmol/L), as determined by 2-color FACS analysis. Results are expressed as mean ± SD of triplicates of 1 representative experiment (out of 3) for each donor (B). Analysis of TCR V gene expression of γδ T cells with specific mAb for Vγ9 and Vδ2 variable genes of the γδ TCR. Bars are mean values ± SD of triplicates of donor S.R. In the 5 other donors tested, the range of Vγ9Vδ2 TCR expression varied from 85% to 95% of all γδ T cells (C).