Fig. 3.
Fig. 3. Proliferative response of purified γδ T cells on stimulation with pamidronate. / Purified PBMC (4 × 104/well) were cultured in the presence of medium (IL-2 alone), IPP (4 μmol/L), pamidronate (4 μmol/L), or PHA (1 μg/mL). Low doses of IL-2 (10 U/mL) were added after 48 hours of culture. After 84 hours, cells were pulsed with [3H] thymidine (1 μCi) for 12 hours. Results are shown as cpm (mean ± SD of triplicate cultures).

Proliferative response of purified γδ T cells on stimulation with pamidronate.

Purified PBMC (4 × 104/well) were cultured in the presence of medium (IL-2 alone), IPP (4 μmol/L), pamidronate (4 μmol/L), or PHA (1 μg/mL). Low doses of IL-2 (10 U/mL) were added after 48 hours of culture. After 84 hours, cells were pulsed with [3H] thymidine (1 μCi) for 12 hours. Results are shown as cpm (mean ± SD of triplicate cultures).

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