Cytolytic response of pamidronate-activated γδ T cells against lymphoma or myeloma targets.

In a standard 4-hour chromium release assay, a pamidronate-induced γδ T-cell line was incubated with the Burkitt lymphoma cell line Daudi, 2 myeloma cell lines (RPMI 8226, U266), or allogeneic PHA-induced peripheral blood leukocyte blasts at indicated E:T ratios in the presence of low-dose IL-2 (10 U/mL). Cytotoxicity is expressed as percentage specific lysis of triplicate cultures.