Fig. 8.
![Fig. 8. Effect of γδ T-cell depletion on pamidronate-induced autologous plasma cell decrease in BMMC cultures. / BMMC of 3 patients with MM (patients 1-3) were cultured under standard conditions (medium with 10 U/mL IL-2) or after depletion of γδ T cells by MACS (patients 1-3 γδ−) in the presence of different pamidronate concentrations (0.4 μmol/L, 4 μmol/L, 40 μmol/L). After 5 days, the number of viable plasma cells was determined as described in “Materials and methods.” Results are expressed as percentage of plasma cells according to the following calculation: [plasma cell number in pamidronate-treated cultures]/[plasma cell number in control cultures (medium alone)] × 100. Each bar represents the mean value ± SD of triplicate cultures.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/2/10.1182_blood.v96.2.384/5/bloo01307008x.jpeg?Expires=1735838359&Signature=ZZ7mJ6D~tradr0W2tAdlhm1VKbl7FhonKj5ZUiotU5R~SNvKVmOUZMbMeY5E5VBMma7mkv16dn2TtyZNizYSNqCUgy289Aq28U1dTXXUakfVc6gmGU-D45Xd3W5P4T5GOKsmz8tsnF1gQjlaXzF3H4wkH6iyqdydtRAKtyNDqf5VubBEU9u4j5qqw1qkzCJHoPjpjCCmBkhiSOkV~UFCo5r~3IYKIt9xpKlAcKzABhDeUwK3R8uGhq8oPq-aOMCsVxwnQqXXIMjer881UsnumGcu1eJh9LwWfDS196qYxvMvzoCFxMEWV~J98Y06~G75Au3pXYKQ628uwwckjnucgA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of γδ T-cell depletion on pamidronate-induced autologous plasma cell decrease in BMMC cultures.
BMMC of 3 patients with MM (patients 1-3) were cultured under standard conditions (medium with 10 U/mL IL-2) or after depletion of γδ T cells by MACS (patients 1-3 γδ−) in the presence of different pamidronate concentrations (0.4 μmol/L, 4 μmol/L, 40 μmol/L). After 5 days, the number of viable plasma cells was determined as described in “Materials and methods.” Results are expressed as percentage of plasma cells according to the following calculation: [plasma cell number in pamidronate-treated cultures]/[plasma cell number in control cultures (medium alone)] × 100. Each bar represents the mean value ± SD of triplicate cultures.
