BCRP and MDR1 mRNA expression in blast cells.
(A) Expression of BCRP mRNA in blast cell samples from 21 patients with acute leukemia, determined by RT-PCR, as described in “Study design.” Data are expressed relative to the expression of BCRP mRNA in MCF-7 cells, after correction for the expression of β-actin mRNA in the sample, and are arranged in the figure in the order of magnitude of BCRP expression. The numbers given in the figure refer to the median values of BCRP expression for each quartile. Patient sample number is a unique identifier for each patient in the study. (B) Expression of MDR1 mRNA in blast cell samples from 9 patients with acute leukemia, determined by RT-PCR, as described in “Study design.” Data are expressed relative to the expression of MDR1 mRNA in HL-60/Vinc cells (× 10−7), after correction for the expression of β-actin mRNA in the sample. (C) Correlation of the expression of MDR1 mRNA with the expression of BCRP mRNA in blast cells from 9 acute leukemia patients. BCRP mRNA expression is reported relative to MCF-7 cells. MDR1 mRNA expression is reported relative to HL-60/Vinc cells (× 10−7).
