Cell surface phenotype comparison of DC and DC–IFN-β generated with 0.1 ng/mL or 1 ng/mL IFN-β.

DC and DC–IFN-β were harvested on day 10 of culture, washed twice, and prepared for flow cytometry as described in Materials and methods. Light lines represent staining of DC generated in the absence of exogenous IFN-β, whereas bold lines indicate staining of DC–IFN-β generated with 0.1 ng/mL or 1 ng/mL IFN-β, as indicated. Unlabeled isotype controls are depicted in upper left corner panels, and direct FITC-conjugated isotype control for CD83 staining is depicted in the panels directly left of CD83. Specificity of the staining with specific mAbs is as indicated below the panels. Results are representative of 5 to 7 independent experiments.