Fig. 5.
![Fig. 5. DC–IFN-β have reduced capacity to induce a mixed lymphocyte reaction. / Naive CD4+ T cells were stimulated with either allogeneic DC or DC–IFN-β (0.1 ng/mL or 1 ng/mL) at the indicated ratios. DC were washed to remove cytokines used for in vitro differentiation. No exogenous cytokines were added to cultures for measurement of [3H]-thymidine incorporation. After 5 days in culture, wells were pulsed with 1 μCi of [3H]-thymidine per well. Twenty-four hours later, plates were harvested and incorporated [3H]-thymidine was measured. Means of triplicate measurements are shown. A representative of 4 independent experiments is shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.210/4/bloo01352005x.jpeg?Expires=1735626931&Signature=CfPep5tEH0bAT~vc9Krh5pXdfUDqcsV8CaR~nbJqgPskgc59n878967i3wcobi5WHOONHXbAeCwc9Kw-3xG2gVEK4ULrUcxYyul3Jizc3gCuEBMqWqYnPUBmXyFBKumZFgaX6dFrN8GwfK2SmuEmjSVJHk9rxRZC4R~LEt~OX4mqkKEyX90FQGMLc~qeU3GOD5jGtNe943L4ImBDZQvrIkFdolJIHmFywqpwZybP82CJh87ADhOS4pvYmht1LtXbqfFytjYaE2bDXm-JLlnRTyAFwoUxQWLWDbnFV8xpB9o3pbkzECqNdNzI~KqL0ICzrQDSDRwkAL5Yhz3dmzvs6A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DC–IFN-β have reduced capacity to induce a mixed lymphocyte reaction.
Naive CD4+ T cells were stimulated with either allogeneic DC or DC–IFN-β (0.1 ng/mL or 1 ng/mL) at the indicated ratios. DC were washed to remove cytokines used for in vitro differentiation. No exogenous cytokines were added to cultures for measurement of [3H]-thymidine incorporation. After 5 days in culture, wells were pulsed with 1 μCi of [3H]-thymidine per well. Twenty-four hours later, plates were harvested and incorporated [3H]-thymidine was measured. Means of triplicate measurements are shown. A representative of 4 independent experiments is shown.
