Fig. 7.
Costimulatory signals provided by DC–IFN-β are insufficient for optimal IFN-γ production by CD4+ T cells.
A total of 5 × 104 monocytes, DC, or DC–IFN-β were cultured with 5 × 105 naive CD4+ T cells and immobilized anti-CD3. APC were washed to remove cytokines used for in vitro differentiation. No exogenous cytokines were added to stimulation cultures. Supernatants were harvested from the primary and secondary stimulations at the indicated time points. IFN-γ secretion (top) and lymphotoxin (LT) secretion (bottom) were measured by ELISA. Results indicate that DC–IFN-β induced IFN-γ production was significantly different from IFN-γ production induced by DC (n = 4; *P < .05 as determined by paired ttest). Results shown are the mean and SEM.
