Comparison of the fluorescence-labeled TCR-β and TCR-γ PCR products by GeneScan analysis.

The x-axes represent molecular size (base pairs) and the y-axes fluorescence intensity. Samples A-D were produced by TCR-β PCR and samples E-H by TCR-γ PCR. (A, E) Normal tonsillar tissue. (B, F) Cutaneous T-cell lymphoma (case 23). (C, G) Psoriasis. (D) T-cell line HUT102 (243 bp). (H) T-cell line HUT102 (205 bp). Note: TCR-β PCR products regularly differ by 3 base pairs, indicating the presence of functional TCR-β rearrangements. TCR-γ PCR products differ by only 1 base pair, indicating frequent out-of-frame rearrangements.